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Twenty grams were blended for 2 minutes with cold chloroform-methanol mixture (2:1 v v -1 ) in proportion of 10 mL of solvent mixture per gram of tissue (except 20 ml of solvent per gram of liver tissue) in a Warring blender

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doi: 10.1111/j.1365-313X.2010.04472.x 74 Garcia-MolinaA.Andrs-ColsN.Perea-GarcaA.NeumannU.DodaniS